8-Hydroxyquinoline

8-Hydroxyquinoline structural formula

CAS Number148-24-3
Molecular FormulaC9H7NO
Molecular Weight145.161
InChI KeyMCJGNVYPOGVAJF-UHFFFAOYSA-N
LogP2.02
Synonyms
  • 8-Hydroxyquinoline
  • Quinolin-8-ol
  • 8-Quinolinol
  • 148-24-3
  • Oxyquinoline
  • 8-Quinolinol
  • 5-21-03-00252
  • 1-Azanaphthalene-8-ol
  • 8-Hydroxychinolin
  • 8-Oxyquinoline
  • Albisal
  • Chinolin-8-ol
  • Fennosan H 30
  • HYDROXYQUINOLINE
  • NSC 2039
  • NSC 285166
  • NSC 402623
  • NSC 48037
  • NSC 54230
  • NSC 615011
  • NSC 82404
  • NSC 82405
  • NSC 82409
  • NSC 82410
  • NSC 82412
  • Oxoquinoline
  • Oxychinolin
  • Phenopyridine
  • Quinolein-8-ol
  • quinoleine-8-ol
  • quinolin-8-ol
  • QUINOLINE, 8-HYDROXY-
  • Quinophenol
  • BRN 0114512
  • Caswell No. 719
  • EINECS 205-711-1
  • EPA Pesticide Chemical Code 059803
  • Fennosan
  • Fennosan HF-15
  • Hydroxybenzopyridine
  • NCI-C55298
  • Oxybenzopyridine
  • USAF EK-794
  • o-Oxychinolin
  • UNII-5UTX5635HP
  • 8-Chinolinol
  • 8-Hydroxy-chinolin
  • 8-OQ
  • 8-Quinol
  • 123574-67-4
  • 24804-14-6

Applications:

HPLC Method for Analysis of 8-hydroxyquinoline

April 30, 2018

Separation type: Liquid Chromatography Mixed-mode




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8-hydroxyquinoline




High Performance Liquid Chromatography (HPLC) Method for Analysis of 8-hydroxyquinoline



8-Hydroxyquinoline, or oxine, is a common chelating agent that also serves as an effective antiseptic and antifungal compound. Since it is an effective chelating agent, it is often used in LC applications to determine metal contamination in silica-based columns. Even small amounts of metal ions on the silica surface can cause significant peak distortion with very low peak efficiency and poor peak symmetry.
8-Hydroxyquinoline can be retained and analyzed on either a Primesep 100 mixed-mode column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. Compared to a standard C19 column, Primesep 100 offers significantly better retention characteristics due to the ionic interaction of basic quinoline molecules with the cation-exchange functional groups of the column’s stationary phase.
This analytical method can be adapted to a Primesep 200 mixed-mode column by replacing the H2SO4 buffer with Phosphoric acid (H3PO4). The analytical method on either column can be UV detected at 200 nm with high resolution and peak symmetry.
For LC-MS applications, the H3PO4 can be substituted with Ammonium Formate (AmFm) with similar results on a Primesep 200 column.

 

Primesep 100 also offers much more retention compare to any C18 column due to ionic interaction of basic quinoline molecule with cation-exchange functional groups of the Primesep 100 stationary phase.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
8-Hydroxyquinoline
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.