| CAS Number | 124505-87-9 |
|---|---|
| Molecular Formula | C17H26N6O4 |
| Molecular Weight | 378.43 |
| InChI Key | AYEKKSTZQYEZPU-RYUDHWBXSA-N |
| LogP | -4.7 |
| Synonyms |
Applications:
HPLC Method for Analysis of Pentosidine
March 16, 2018
HPLC Method for Pentosidine on Obelisc R by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pentosidine
Pentosidine is a advanced glycation end-product (AGE) with C17H26N6O4 • CF3COOH chemical formula. Since it is a fluorescent biomarker, it is often used as an indicator in studies of aging and chronic diseases including diabetes and uremia.
Pentosidine can be retained and analyzed using the Obelisc R stationary phase column. The analysis utilizes an isocratic method with a simple mobile phase consisting of water and acetonitrile (MeCN) with an ammonium acetate buffer. Detection is performed using UV.
| Column | Obelisc R, 4.6 x 150 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | MeCN – 20% |
| Buffer | Ammonium acetate |
| Flow Rate | 1.0 ml/min |
| Detection | UV |
| Class of Compounds | Advanced Glycation End-Product |
| Analyzing Compounds | Pentosidine |
Application Column
Obelisc R
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Application Analytes:
PentosidineApplication Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
