HPLC Analysis of Alachlor

 

 

Alachlor is a chloroacetanilide family herbicide which inhibits parts of the gibberellin pathway. The EPA classifies alachlor as slightly toxic and set the maximum contaminant level at 2 ppb. The EU banned alachlor in 2006. Obelisc R was used to retain alachlor and separate it from impurities. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.

Condition

 
Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Alachlor

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Alachlor

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.