2,4-D makes up half of the formula for Agent Orange, the defoliant that was used during the Vietnam War and Malayan Emergency. Commonly used in lawn and turf pesticides, 2,4-D is typically mixed with other pesticides such as mecoprop and dicamba. It was analyzed on Primesep 100 and Obelisc R. Primesep 100 separates using reverse-phase and acidic ion-pairing groups. Obelisc R has many modes of separation which allow it to tune retention. Method is LC/MS compatible and conditions could be replicated to retain many pesticides.