Alachlor Separation on Obelisc and Primesep HPLC Columns

Alachlor is a herbicide which inhibits the gibberellin pathway. Use of alachlor is banned by the European Union and the U.S. EPA. The EURL (European Union Reference Laboratory) includes alachlor in a pesticide analysis of teas and chamomile by liquid and gas chromatography. A single multiresidue method was developed using both Primesep B2 and Obelisc R analyze many pesticides. Obelisc R contains embedded ionic and hydrophobic groups which can assist in fine tuning separations, while Primesep B2 contains embedded basic ion-pairing groups. Method is LC/MS compatible and can be used as a general approach for analyzing alachlor.