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HPLC Application of Amino Acids Analysis in Acid Gradient Condition  11 underivatized amino acids (aspartic acid, glutamic acid, alanine, valine, methionine, isoleucine, cysteine, phenylalanine, histidine, lysine, and arginine) are separated by a Primesep 100 HPLC column by reversed-phase and ion-exchange mechanisms with LC/MS compatible conditions without the use of ion-pair reagents. The HPLC separation uses a TFA (trifluoroacetic acid) gradient in a mobile phase of water acetonitrile (MeCN, ACN with evaporative light scattering detection (ELSD). Application Analytes: Alanine Amino Acids Arginine Aspartic Acid Cysteine Glutamic Acid Histidine Isoleucine Lysine Methionine Phenylalanine Valine Application Detection: UV Detection |
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