Amino Sugars Analysis in Ion Exchange and Reverse Mode

Primesep 100 retains an amino sugar by a combination of cation exchange and reversed phase. Columns with reversed-phase functionality alone are unable to retain this amino sugar. The polar interactions of the Primesep 100 column together with the reversed-phase interactions retain the amino sugar and resolve it from its many impurities. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and sulfuric acid (H2SO4) with UV detection at 205 nm.

Condition

Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H2SO4
Flow Rate 1.5 ml/min
Detection UV 205 nm

 

Description

Class of Compounds
 Hydrophilic, Ionizable
Analyzing Compounds Sugars

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amino Sugar

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.