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HPLC Application of Complex Mixture of Acids, Bases, Amino Acids, and Neutral Compounds  Primesep 100 separates a mixture of amino acids (tyrosine, phenylalanine), organic acids (benzoic acid, mandelic acid), amines (benzylamine, pyridine), and neutrals (benzonitrile, toluene) in one HPLC run by combining reversed-phase, cation-exchange, and polar interactions. The method is tunable and peak order can be changed significantly by adjusting acetonitrile and trifluoroacetic acid concentrations. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA) and compatible with UV, mass spec (LC/MS) and evaporative light scattering (ELSD) detection. Application Analytes: Amino Acids Benzoic Acid Benzonitrile Benzylamine Mandelic Acid Phenylalanine Pyridine Toluene Tyrosine Application Detection: UV Detection |
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