Dewetting Study in Aqueous Conditions

Most reversed-phase HPLC columns, such as C18, are incompatible with 100% aqueous mobile phases due to dewetting (collapse) of the stationary phase. Primesep columns combine reversed-phase with polar functional groups which eliminate the problems of dewetting. The Primesep 100 column in this application with an acetonitrile (MeCN, ACN) shows a retention time of 2.5 minutes. In 100% water, the retention time is 3 minutes even after sitting 24 hours in the water mobile phase. On a C18 phase, the analyte retention would have been lost and elution at the void (1.5 minutes) would have resulted.

Condition

Column Primesep 100
Mobile Phase MeCN/H2O
Buffer No
Flow Rate 1.0 ml/min
Detection UV, 270 nm

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Benzoquinone

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.