Effect of Concentration of Organic Modifier on Retention of Cation and Anions

Primesep 100 and Primesep B columns connected in series allow the quantitation of sodium chloride and potassium bromide ions in one injection. The Primesep B column retains the chloride and bromide anions by anion exchange, and the Primesep 100 retains the sodium and potassium cations by cation exchange. The retention can be adjusted by changing the water/acetonitrile ratio in the mobile phase. The separation uses a mobile phase mixture of water, acetonitrile (MeCN, ACN) and ammonium acetate with evaporative light scattering detection (ELSD).

Condition

Column Primesep 100, Primesep B , 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 5.0
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Ions,  Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sodium, Potassium,  Bromide, Chloride

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Bromide
Chloride
Potassium
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.