HPLC Analysis of Apple Extract using Mixed-Mode Chromatography

Apple extract is a complex mixture of polyphenols, vitamins and amino acids. Analysis of this mixture is performed by mixed-mode chromatography. Different selectivity is achieved on two different columns: Primesep 200 reversed-phase cation-exchange column, and Primesep D reversed-phase anion-exchange HPLC column. Compounds are monitored by UV detection. Methods are compatible with LC/MS and preparative chromatography and can be used for identification and isolation of specific compounds.