HPLC Analysis of Apple Extract using Mixed-Mode Chromatography


Apple extract is a complex mixture of polyphenols, vitamins and amino acids. Analysis of this mixture is performed by mixed-mode chromatography. Different selectivity is achieved on two different columns: Primesep 200 reversed-phase cation-exchange column, and Primesep D reversed-phase anion-exchange HPLC column. Compounds are monitored by UV detection. Methods are compatible with LC/MS and preparative chromatography and can be used for identification and isolation of specific compounds.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Apple Extract

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.