HPLC Analysis of Clopyralid

Clopyralid (3,6-dichloro-2-pyridinecarboxylic acid) is a selective herbicide effective against broad-leaf plants such as clovers and thistle. A single residue method for the EURL (European Union Reference Laboratory) was developed for clopyralid and other acidic compounds using QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) methodology. Retention of clopyralid was controlled on both Obelisc R and a 3 micron Primesep 100 column of the same dimension. This method is LC/MS compatible and useful for separating many pesticides.

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min,  7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min,  7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
 Insecticide, Herbicide, Fungicide,  Hydrophobic, Ionizable
Analyzing Compounds Clopyralid

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Clopyralid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.