HPLC Analysis of MCPA on Primesep and Obelisc Mixed-Mode Columns

 

 

MCPA (4-Chloro-2-methylphenoxy)acetic acid) was synthesized to control broad-leaf weeds such as thistle and dock. MCPA forms complexes with metal ions and increase their bioavailability. A single residue method was developed and used by the EURL (European Union Reference Laboratory) for acidic pesticides. MCPA was separated by both Obelisc R and Primesep 100. Primesep 100 is a reverse-phase column with embedded acidic ion-pairing groups while Obelisc R is a mixed-mode column with separates using ionic groups and a long hydrophobic chain. Methods are LC/MS compatible and able to be used with many pesticides.

 

Condition 1

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 25-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 30-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 2

Column Obelisc R, 2.1×150 mm, 5 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Condition 3

Column Primesep 100, 2.1×150 mm, 3 µm, 100A
Mobile Phase Gradient MeCN – 10-70%, 15 min, 7 min hold
Buffer Gradient AmAc pH 3.0- 20-60 mM, 15 min, 7 min hold
Flow Rate 0.4 ml/min
Detection UV, 270 nm

 

Description

Class of Compounds
Synthetic plant hormone, Acid,   Hydrophobic, Ionizable
Analyzing Compounds MCPA

 

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
MCPA
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.