HPLC Retention of Glucosamine

Glucosamine is an amino sugar that is used as precursor in synthesis of glycosylated proteins and peptides. Glucosamine is also a component of several medications, example being oral glucosamine being is used to treat arthritis. It is a very polar compound with no UV activity and pKa of 11. Compound is not retained on modern HPLC columns without ion-pairing reagents. It is often required to quantitate glucosamine in complex matrices with LC/MS detection, making the use of ion-pairing reagents impossible. In this HPLC application glucosamine is retained by cation-exchange mechanism on Primesep 100 mixed-mode column. Retention of glucosamine is controlled by buffer nature, buffer concentration and buffer pH. Method can be used for fast determination of glucosamine in mixtures with proteins, sugars and sugar phosphates. ELSD and LC/MS are detection of choice for polar non-UV active compounds.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Glucosamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.