HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200

 

Application Notes: Methylhistidines are polar amino acids with very similar structures. Methylhistidines are methylated derivative of histidine found in actin. N-methylhistidine is released into urine after the breakdown of actin and myosin. Urinary output of N-methylhistidine is a reliable index of the rate of myofibrillar protein breakdown in musculature. The method for separation of 1-methylhistidine and 3-methylhistidine on Primesep 200 column was developed with LC/MS compatible conditions. This method can be used for quantitation of methylhystidines in urine and other biofluids.

Application Columns: Primesep 200
Application compounds: 1-Methylhistidine, 3-Methylhistidine
Detection technique: UV, LC/MS, ELSD/CAD

 

Condition

Column Primesep 200, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 20/80%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Amino acid , Acid, Hydrophilic, Ionizable
Analyzing Compounds 1-Methylhistidine, 3-Methylhistidine

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
1-Methylhistidine
3-Methylhistidine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.