HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids on Primesep D HPLC Column

Nitrophthalic acid exist in the form of two isomers: 3-nitrophthalic and 4-nitrophthalic acid. Both forms have similar hydrophobicity and ionic properties. Isomers of nitrophthalic acid are separated on Primesep D column by combination of reverse phase and anion-exchange mechanisms. Method developed on a short-50 mm- column and allows fast analysis of these two isomers.