HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids


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Condition

Column Newcrom BH, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O – 60/40%
Buffer H3PO4 – 0.5%
Flow Rate 0.5 ml/min
Detection UV 275nm

Description

Class of Compounds Acid
Analyzing Compounds 3-Nitrophthalic and 4-Nitrophthalic Acids


Nitrophthalic acid exist in the form of two isomers: 3-nitrophthalic and 4-nitrophthalic acid. Both forms have similar hydrophobicity and ionic properties. Isomers of nitrophthalic acid are separated on Primesep D column by combination of reverse phase and anion-exchange mechanisms. Method developed on a short-50 mm- column and allows fast analysis of these two isomers.

Application Column

Newcrom B

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
3-Nitrophthalic Acid
4-Nitrophthalic Acid

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.