HPLC Separation of Active Compounds in Drug Formulation

HPLC Separation of Active Compounds in Drug Formulation


An HPLC method for the separation of active drug compounds on a Primesep 200 column. The retention of compounds is achieved through reverse-phase, cation exchange and hydrophobic interactions. Benzoic acid, hyoscyamine sulfate and phenyl salicylate are baseline separated using simple mobile phases of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) with a UV detector.

Condition

Column Primesep 200, 3.0×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer TFA
Flow Rate 1.0 ml/min
Detection UV, 220 nm

 

Description

Class of Compounds
Acid, Hydrophilic, Ionizable
Analyzing Compounds Benzoic acid, Hyoscyamine Sulfate, Phenylsalicylate)

 

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzoic Acid
Hyoscyamine Sulfate
Phenylsalicylate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.