HPLC Separation of Aminobiphenyls

Aminobiphenyls consist of isomers that differ only in the location of a primary amine group on an aromatic ring. 2-, 3-, and 4-aminobiphenyl are separated on a Primesep D with good peak shape and a short retention time by a mixture of reversed-phase and ion-exclusion interactions. Peak order can be reversed for 2- and 3-aminobiphenyls by using ammonium formate buffer without pH adjustment. The HPLC separations use a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate buffer or acetic acid (HOAc) with UV detection at 250 nm.

Condition

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer Formic Acid, AmFm
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Hydrophobic, Ionizable
Analyzing Compounds 2-Aminobiphenyl, 4 -Aminobiphenyl, 3 -Aminobiphenyl

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
2-Aminobiphenyl
3-Aminobiphenyl
4-Aminobiphenyl

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.