Boronic acid is alkyl, or aryl substituted, boric acid. Boronic acid can form complexes with sugars, amines, and amino acids. Four aromatic boronic acids were separated on a Primesep P mixed-mode reversed-phase cation-exchange column. Primesep P column also has an aromatic fragment which provides pi-pi interaction. Compounds can be monitored by UV, ELSD, CAD and LC/MS. Various mobile phases can be employed for HPLC analysis of boronic acids.
Column | Primesep P, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O |
Buffer | H2SO4 |
Flow Rate | 1.0 ml/min |
Detection | UV 270 nm |
Class of Compounds |
Drug, Acid, Hydrophobic, Ionizable |
Analyzing Compounds | Chlorohenyl-4-boronic acid, Naphtalene-1-boronic acid, Biphenyl-4-boronic acid, 4-(1-naphthyl)phenylboronic acid |
Application Column
Primesep P
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select options4-Chlorophenylboronic acid
Biphenyl-4-boronic acid
Naphthalene-1-boronic acid