HPLC Separation of Benzalkonium Chloride BCT 8358 on Primesep D column

chr_289.gif

Benzalkonium chloride is a mixture of alkylbenzyldimethylammonium chlorides of various even-numbered alkyl chain lengths. It is used as a surfactant. Hydrophobic quaternary amines, like BTC, produce poor peak shape in reversed-phase chromatography due to interaction of quaternary amines with residual silanols. Ideal peak shape can be achieved on reversed-phase anion-exchange columns like Primesep D. Primesep D has a basic group on the surface of silica gel that shields silanols from interacting with quaternary, tertiary, secondary and primary amines. This allows to achieve symmetrical peaks for hydrophobic basic analytes. Compounds need to be hydrophobic enough to get compensation for repulsion effect coming from stationary phase. BTC can be monitored by ELSD and UV. Primesep D will also retain acidic counter-ions of quaternary amines, so simultaneous analysis of hydrophobic basic and hydrophilic basic compounds is possible.

Condition

Column Primesep D, 4,6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection 210

 

<

Description

Class of Compounds
 Surfactant,  Hydrophobic, Ionizable
Analyzing Compounds Benzalkonium Chloride BCT 8358

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Benzalkonium chloride

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.