HPLC Separation of Carboxylic Acids


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Condition 1

Column Newcrom  B, 3.2×50 mm, 3 µm, 100A
Mobile Phase MeCN/H2O- 30/70%
Buffer H3PO4 – 0.5%
Flow Rate 0.5 ml/min
Detection UV, 275 nm

 


HPLC separation of carboxylic acids (nicotinic acid, benzoic acid and acetylbenzoic acid) on Primesep B2 column using an isocratic method by reverse-phase and anion-exchange mechanisms. The elution of carboxylic acids using the same isocratic method and mobile phase can be reversed by using a reverse-phase cation-exchange mechanisms of the Primesep 100 column. The mobile phase is water, acetonitrile (MeCN, ACN) and formic acid with a UV detector.

Condition 2

Column Primesep B2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection UV, 250 nm

 

Description

Class of Compounds
Acid, Hydrophilic
Analyzing Compounds Nicotinic acid, Benzoic acid, Acetylbenzoic acid,

1.4-acetylbenzoic Acid

 

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Acetylbenzoic Acid
Benzoic Acid
Nicotinic Acid/Niacin (3-pyridinecarboxylic acid)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.