HPLC Separation of Cetylpyridinium

Cetylpyridinium Chloride is an antiseptic agent used in mouthwash or dental caries for oral and throat care. Primesep D retains cetylpyridinium by a combination of reversed-phase and ion-exchange mechanisms. The anion-exchange properties of the column can be adjusted by the TFA concentration in the mobile phase and the reversed-phase properties are adjusted by the percentage of acetontrile. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm.