HPLC Separation of Citrulline and Arginine on Primesep 200 Column

 

 

 

 

 

 

 

 

Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.

Condition
Column Primesep 200,  4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
 Ions,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Citrulline, Arginine

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Arginine
Citrulline
Sodium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.