HPLC Separation of Cyclodextrin Sulfobutyl Ether and Hydrophobic Compound

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Cyclodextrin sulfobutyl ether is sulfonated derivative of cyclodextrin. The degree of sulfobutylation varies, and the compound is a mixture of several components. All compounds are very polar and acidic in nature, and are not retained by traditional reversed-phase chromatography. Separation was achieved on an Obelisc N column in anion-exchange mode. Hydrophobic naphthalene was used a a reference standard. Cyclodextrin was monitored by ELSD.

Condition

Column Obelisc N2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH, THF
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection ELSD, 60C UV 270 nm

 

Description

Class of Compounds
Hydrophobic, Ionizable
Analyzing Compounds Sodium, Beta Cyclodextrin Sulfobutyl Ether, Naphthalene

 

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

Select options
Application Analytes:
Betadex Sulfobutyl Ether Sodium

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.