HPLC Separation of Cytidines


Cytidine phosphates are nucleotides. Nucleotides contain a nucleobase, five-carbon sugar and one or three phosphate groups. All three cytidines are very polar and cannot be retained by reversed-phase chromatography without a ion-pairing reagent. Mixed-mode Primesep SB column easily allows to retain and separate mono-, di-, and tri-phospates by anion-exchange mechanism. Other nucleotides can be separated as well by this approach. Nucleotides in biofluids can be analyzed directly on this column without any significant sample preparation. Most of the proteins from biological fluids will be repelled from the surface of silica gel. Method is compatible with UV, ELSD, CAD and LC/MS.

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Cytidine Diphosphate
Cytidine Monophosphate
Cytidine Triphosphate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.