HPLC Separation of Epinephrine and Related Impurities

Epinephrine (also referred to as adrenaline) is a hormone and neurotransmitter. It is a catecholamine, a sympathomimetic monoamine derived from the amino acids phenylalanine and tyrosine. Epinephrine is polar basic compounds and it is retained on mixed-mode cation exchange columns without ion-pairing reagent. Epinephrine is retained by weak reversed phase and strong cation-exchange mechanisms. Formulations for epinephrine might have citric and ascorbic acid and EDTA. These three compounds are not retained by cation-exchange or reversed-phase mechanisms and elute in the void. Current HPLC method can be used for quantitation of epinephrine and recepinephrine (recemate) in various compositions. Epinephrine and related impurity can be monitored by UV, ELCD/MS, ELSD or Corona CAD. Corresponding buffer is required for each detection technique.

Condition

Column Primesep 200,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection 410

 

Description

Class of Compounds
Ions,  Hydrophilic, Ionizable
Analyzing Compounds Ascorbic acid, Citric acid, EDTA, Racepinephrine

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Epinephrine
Racepinephrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.