HPLC Separation of Histamine and Phenethylamine


Histamine is a basic compound that acts like neurotransmitter. Phenethylamine is a natural amine alkaloid psychoactive compound with stimulant effects. It forms by enzymatic decarboxylation of phenylalanine, and also acts as neurotransmitter. Both compounds are separated in one HPLC run on Primesep C and Primesep 200 columns by reversed-phase cation-exchange mechanism. Primesep C provides shorter retention time and histamine and phenethylamine can be separated within 5 minutes. Primesep C and Primesep 200 columns do not require ion-pairing reagent as it is attached to the surface of Primesep silica gel. Method can be used for analysis of these two compounds in plasma, blood and urine with UV, ELSD, CAD or LC/MS detection.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Histamine
Phenethylamine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.