HPLC Separation of Hydrocortisone and Hydrocortisone’s Degradation Products


Cortisol (hydrocortisone) is a steroid hormone produced by the adrenal gland. Hydrocortisone is a hydrophobic neutral compound with some polar groups in its structure. Several Primesep mixed-mode columns were used for separation of hydrocortisone and its degradation products. Each column showed a different selectivity for separation, which attributed to different polar-embedded groups on Primesep 100 and Primesep D columns. Both columns can be used for degradation studies of various hydrophobic compounds.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
HPLC Method for Analysis of Hydrocortisone
Hydrocortisone

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.