HPLC Separation of m-Cresol Purple and Related Impurities

m-Cresol purple is indicator dye for spectrophotometric pH measurements. These measurements are widely used in oceanic CO2 research. Purity of the indicator is a critical issue in accurate determination and can cause uncertainty in pH measurement. Numerous attempts to purify m-cresol purple failed due to lack of separation or low loadability of HPLC columns. Primesep B2 method separates 4 major impurities from main compound. All impurities are well separated. m-Cresol Purple is hydrophobic and acidic in nature. Parent compound and impurities are retained based on reverse phase and anion-exchange mechanisms. Method can be used for high loadabiity preparative separation of acidic dyes.

Application Column

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
m-Cresol Purple

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.