HPLC Separation of Norepinephrine on Legacy L1 Column

Norepinephrine was analyzed based on the United States Pharmacopeia. The Legacy L1 HPLC column is classified as a reversed-phase column according to USP manual. This column can be used for other methods that require this type of column. Legacy L1 can also be used instead of columns from other column manufacturers.

Condition

Column Legacy L1, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeOH/H2O – 60/40%
Buffer Sodium heptanesulfonate – 0.22%
Flow Rate 1.0 ml/min
Detection UV, 280 nm

 

Description

Class of Compounds
Drug, Neurotransmitter, Hydrophobic, Ionizable
Analyzing Compounds Norepinephrine

 

Application Column

SIELC Legacy L1 HPLC column

Legacy L1

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Select options
Application Analytes:
Norephedrine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.