HPLC Separation of Nucleobases (2)


Nucleosides consisting of deoxyribose and a nucleobase cannot be separated on a common C18 column. By using a Primesep 200 mixed-mode column with a cation-exchange mechanism, nucleosides: deoxyuridine, deoxyguanosine, deoxycytidine and deoxyadenosine, can be baseline separated in a short time using an isocratic method with a simple mobile phase of water, acetonitrile (MeCN, ACN) and TFA as buffer. UV detection at 270nm.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Deoxyadenosine
Deoxycytidine
Deoxyguanosine
Deoxyuridine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.