HPLC Method for Separation of Serine, a-Methylserine, GABA, and Methionine Mixture on Primesep 100 column

HPLC Method for Separation of Serine, a-Methylserine, GABA, and Methionine on Primesep 100 Column


Four underivatized amino acids (serine, methylserine, GABA and methionine) were separated on a Primesep 100 reversed-phase cation-exchange mixed-mode HPLC column. Two methods, one for UV and one for ELSD/LC/MS show good separation and peak shape for underivatized amino acids. This column and general HPLC approach can be used for analysis of underivatized amino acids. Primesep 100 is designed to replace reversed-phase HPLC column in combination with ion-pairing reagents.

ColumnPrimesep 100, 4.6×250 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O
BufferH3PO4 or Formic acid
Flow Rate1.0 ml/min
DetectionUV, 200 nm, ELSD
Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing CompoundsSerine, Methylserine, GABA, Methionine

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Methionine
Serine
a-Methylserine
gamma-Aminobutyric Acid (GABA)

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.