HPLC Separation of Tetracyclines


Tetracyclines are a group of broad spectrum of antibiotics. Structure can be described as derivatives of polycyclic naphthacene carboxamide. Tetracyclines are hydrophilic and basic compounds due to the presence of hydroxyls and amino group. Mixture of tetracyclines and their impurities were separated based on weak reversed-phase and strong cation-exchange mechanisms on a Primesep 200 mixed-mode HPLC column. Elution of compounds was monitored by UV.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Tetracycline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.