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HPLC Application of HPLC Separation of Toluidine Isomers  Toluidine isomers are based on toluene and differ whether the primary amine is ortho, meta or para to the methyl group. A Primesep 200 column with an ammonium formate buffer or a Primesep 100 column with sodium phosphate baseline resolves the three regioisomers. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), ammonium formate, or sodium phosphate and ultraviolet (UV) detection at 250 nm. Application Analytes: m-Toluidine o-Toluidine p-Toluidine Application Detection: UV Detection ELSD/MS Detection |
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