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HPLC Application of HPLC Separation of Vitamin C and Vitamin B2  Vitamin C (ascorbic acid) and Vitamin B2 (riboflavin) are water-soluble vitamins that are not stored in the body and must be replaced each day. Primesep D easily resolves Vitamins C and B2 and the elution order can be reversed by adjusting acetonitrile and TFA concentrations in the mobile phase. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and trifluoroacetic acid (TFA) and UV detection at 250 nm. Application Analytes: Vitamin B2 (Riboflavin) Vitamin C Application Detection: UV Detection |
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