HPLC Separation of Vitamin E and Vitamin E Acetate on Primesep B Column

Primesep B separates Vitamin E and Vitamin E acetate with reversed peak order of a traditional C8 column. The different selectivity is due to an embedded basic functional group on the stationary phase which contributes polar interactions to the separation. Excellent peak shape results with a mass spec compatible mobile phase of water and acetonitrile (MeCN, ACN) with UV detection at 205 nm.

Condition

Column Primesep B, 3.2×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 90/10%
Buffer
Flow Rate 0.5 ml/min
Detection UV 205

Description

Class of Compounds Vitamins, Hydrophilic, Ionizable
Analyzing Compounds Vitamin E,  Vitamin E Acetate

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Vitamin E
Vitamin E Acetate

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.