Loading Study for Preparative Chromatography

Ion-pairing reagents are not compatible with preparative chromatography, LC/MS, and ELSD detection. Primesep columns offer an alternative way to retain polar compounds through an ion-exchange mechanism with embedded ion-pairing groups. The amount of ion-pairing groups on Primesep columns is comparable to the amount of ion-pairing groups on regular ion-exchange columns. In many instances, reverse phase columns designed for retention of polar compounds do not offer enough capacity when a sample has a high ionic strength; however Primesep columns have a high capacity for ion-exchange retention. This high capacity results in high sample loading on this Primesep 100 column and is one of the most important characteristics of stationary phases in scale-up separations.

Application Column

Primesep 100

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.