Peak Symmetry Study with Amitryptiline

Primesep B has been applied to the analysis of amitriptyline. Amitriptyline is a tertiary amine commonly used a probe in reversed-phase columns to test silanol activity. On C18 phases amitriptyline often tails unless mobile phase modifiers such as triethylamine or high ionic strength buffers are used. Primesep B has an embedded cation which shields the silica by ionic repulsion with the amitriptyline cation. Excellent peak shape results with a mass spec compatible mobile phase of water, acetonitrile (MeCN, ACN) and trifluoracetic acid (TFA).

Application Column

Primesep B

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amitriptyline

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.