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HPLC Application of Primesep C Column Retention Control  Primesep C separates benzylamines with an easily tunable HPLC method that allows retention control. Benzylamine, N-methylbenzylamine, and N,N-dimethylbenzylamine peak order and retention can be significantly changed by altering the water/acetonitrile ratio in the mobile phase which changes the combination of cation exchange, complex formation, and hydrophobic interactions. This mixture is separated with a mobile phase of water, acetonitrile (MeCN, ACN) and triethylamine (TEA) phosphate with UV detection at 210 nm. Application Analytes: Benzylamine N,N-Dimethylbenzylamine N-Methylbenzylamine Application Detection: UV Detection |
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