Retention of Sialic Acid on Primesep D Column

Sialic acid is a nitrogen or oxygen substituted neuraminic acid. It is a sugar-based derivative with very polar properties. The N-substituted compound is acidic in nature. Sialic acid has no retention in reversed-phase, unless an ion-pairing reagent is used. The method for analysis of sialic acid was developed on the Primesep D reversed-phase anion-exchange column. The method is compatible with ELSD and LC/MS and also can be used for the analysis of sialic acid in biological fluids like blood, serum, urine, etc. Retention time of sialic acid is controlled by the pH of the mobile phase and the buffer concentration. It is retained by very weak, reversed-phase and weak, anion-exchange mechanisms.

 

Condition

Column Primesep D, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sialic Acid

 

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Sialic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.