Separation of Small Lysine-based Peptide Oligomers on BIST B+ Column

 

HPLC Method for Analysis of Polylysine on BIST™ B+ Column

Polylysine includes a large group of similar polymers with various uses. Some are used as food preservatives, while others are used for drug delivery in pharmaceuticals. Polymers with charged monomeric units, such as polylysine, are often difficult to separate using typical ion-exchange chromatography due to very strong and often irreversible interactions with the oppositely charged column surface. Therefore, an extremely high concentration of the buffer, up to several molar, is usually needed to facilitate an ion-exchange process. This high buffer concentration, however, is not desirable because of the significantly increased viscosity of the mobile phase and the salt formation in the pump components. With BIST™, these polymers can be separated and retained with relatively weak buffers (in the mM regime) and a fairly simple gradient. Using this new and unique analysis method, polylysine can be retained and UV detected at 215 nm.

Condition

Column BIST B+, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 215 nm

 

Description

Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Lysine-based Peptide Oligomers

 

Application Column

BIST B+

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Lysine
Polylysine

Application Detection:
ELSD Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.