Applications  →  Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column

HPLC Application of Separation of Steroids in Reverse and Normal Mode on Primesep 100 Column



The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both reversed- and normal-phase modes. Switching modes by changing the mobile phase reverses peaks 2 and 3. The separation method uses a mobile phase mixture of water and acetonitrile (MeCN, ACN) for reversed phase and hexane and ethanol for normal phase with UV detection at 240 nm.

Application Analytes:

Steroid

Application Detection:

UV Detection
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