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HPLC Application of Separation of Sulphonium Ions in Polar Organic Mode  The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD). Application Analytes: Methanesulfonic Acid Methoxydimethylsulphonium Sulfonium Ions Trimethylsulphonium Application Detection: ELSD/MS Detection |
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