Simultaneous Analysis of Glucose, Sodium, Potassium and Calcium Ions

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Neutral sugar, monovalent and divalent cations were separated in HILIC/cation-exchange mode on a Primesep N mixed-mode column. Mobile phase is compatible with LC/MS and method can be used for analysis polar molecules in HILIC, cation-exchange or anion-exclusion modes.

Condition

Column Primesep N, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc pH 4.0
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

Description

Class of Compounds
Ions, Hydrophilic, Ionizable
Analyzing Compounds Potassium, Sodium, Calcium, Glucose

 

Application Column

Primesep N

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Calcium
Potassium
Sodium

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.