Simultaneous Separation of Melamine and Cyanuric Acid

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Melamine is an organic basic compound that is derived from cyanamide. It contains 66% nitrogen by mass. Addition of melamine is used to increase protein count in milk products. Cyanuric acid is a trimer of cyanic acid. Melamine and cyanuric acid can form a insoluble complex. Both compounds has been linked as additives to modify nitrogen count in products. Cyanuric acid is a slightly acidic hydrophilic compound, while melamine is a basic hydrophilic compound. Both compounds were separated on Obelisc R reversed-phase ion-exchange column with LC/Ms compatible mobile phase. Method can be used for generic analysis of basic and acidic compounds with LC/MS compatible conditions.

 

Condition

Column Obelisc N, 4,6×150 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD 50C

 

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Description

Class of Compounds
 Acid,  Hydrophobic, Ionizable
Analyzing Compounds Melamine,  Cyanuric Acid

Application Column

Obelisc N

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Cyanuric Acid
Melamine

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.