|
|
SIELC Applications Sorted By Column Type
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 100 stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep 100 columns. Primesep 100 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 100 separates underivatized amino acids and amines by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 200 stationary phase combines a hydrophobic chain and a weakly acidic functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep 200 columns. Primesep 200 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 200 separates underivatized amino acids, amines, isomers and structurally related compounds by combining reversed phase and ion exchange. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids and diacids are separated by reversed phase and ion exclusion. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep 500 stationary phase combines a hydrophobic chain and a weak carboxylic acid functional group with a pKa of 5 as a cation exchanger. Acids, bases, and neutrals can be retained and separated by Primesep 500 columns. Primesep 500 retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep 500 retains basic compounds by reversed phase at mobile phase pH < 5 and separates acids by anion exclusion at mobile phase pH > 5. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep A stationary phase combines a hydrophobic chain and a strong acid functional group as a cation exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep A columns. Primesep A retains hydrophobic compounds by reversed-phase retention alone or a combination of reversed-phase and ion-exchange retention. Primesep A separates underivatized amino acids, amines and weak bases by combining reversed-phase and ion-exchange mechanisms. In this case the acidic functional group acts as an embedded ion-pairing reagent. Organic acids are separated by reversed phase and ion exclusion. Inorganic cations can be separated by cation exchange. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep AB stationary phase combines a hydrophobic chain and both acidic and basic functional groups as ion exchangers. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep AB columns. Primesep AB separates bases by cation exchange and acids by anion exchange. Polar compounds such as acids and bases can be separated in one method. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep B stationary phase combines a hydrophobic chain and a strongly basic functional group as an anion exchanger recommended for mobile phase pH's of 1.5 to 4. Primesep B separates bases by an ion-exclusion mechanism. The basic functional group acts as an embedded ion-pairing reagent. Recommended buffers for Primesep columns are trifluoroacetic acid (TFA), phosphoric acid (H3PO4), perchloric acid (HClO4), and formic acid. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep B2 stationary phase combines a hydrophobic chain and a basic functional group as an anion exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep B2 columns. Primesep B2 also separates bases by an ion-exclusion mechanism. Primesep B2 demonstrates significant improvement in retention and selectivity for the separation of weak acid analytes by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Primesep B2 column is one of the best separation media for natural product extracts. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep B4 stationary phase combines a short hydrophobic chain and a basic functional group as an anion exchanger. Acidic and basic surfactants are separated by a multi-mode mechanism. Primesep B4 also separates hydrophobic bases by an ion-exclusion mechanism. Primesep B4 demonstrates significant improvement in retention and selectivity for the separation of weak acid analytes by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with reversed-phase, ion-exchange and host-guest complex formation properties. The Primesep C stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. Acids, bases, and neutrals can be retained and separated by Primesep 100 columns. Primesep C retains hydrophobic compounds by a reversed phase. Strongly polar and basic compounds such as quaternary amines are retained without the use of ion-pairing reagent by reversed phase and ion exchange. An unusual elution order compared to other HPLC columns is found for primary, secondary, and tertiary amines. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a reversed-phase HPLC column with general hydrophobic and ion-exchange properties for direct injection of plasma samples. The Primesep D stationary phase combines a hydrophobic chain and a basic functional group as an anion exchanger. Primesep D excludes high molecular weight plasma compounds while retaining hydrophobic and acidic small molecules. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a normal-phase HPLC column with embedded acidic groups with a pKa of about 5. The Primesep N stationary phase retains basic compounds by cation exchange at pH > 5. Primesep N separates polar compounds by a HILIC (hydrophilic liquid chromatography) mechanism. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with hydrophobic aromatic and ion-exchange properties. The Primesep P stationary phase combines a phenyl ring and an acidic functional group as a cation exchanger. The phenyl ring separates aromatic compounds by differences in pi-pi interaction. Primesep P retains basic compounds by a cation-exchange mechanism. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a reversed-phase HPLC column with embedded aromatic and ion-exchange groups. The Primesep PB stationary phase combines an aromatic group and a basic functional group as an anion exchanger. The aromatic group separates aromatic analytes by their differences in pi-pi interactions. Neutrals are separated by a reversed phase. Primesep PB separates acids by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a proprietary reversed-phase HPLC column designed for the separation of strong bases while providing good peak shape. Primesep Q separates quaternary amines such as paraquat and diquat. Neutral hydrophobic compounds are separated by reversed phase. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties. The Primesep SB stationary phase combines a hydrophobic chain and a strongly basic functional group as an anion exchanger. Acids, bases, zwitterions and neutrals can be retained and separated by Primesep SB columns. Primesep SB is ideal to retain hydrophobic compounds by reversed-phase retention due to its C18 carbon chain. Basic hydrophobic compounds are usually retained less on Primesep SB columns than on similar RP columns due to a charge repulsion effect. Primesep SB also separates bases by an ion-exclusion mechanism. Primesep SB demonstrates significant improvement in retention and selectivity for the separation of weak acid analytes by a combination of reversed phase and anion exchange. In this case the basic functional group acts as an embedded ion-pairing reagent. Primesep SB column is one of the best separation media for natural product extracts. Methods can be developed on Primesep columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties for the separation of small and medium peptides under 3 kDa and PI value below 7.0. The Promix AP stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. The acidic functional group acts as an embedded ion-pairing reagent. Promix AP provides enhanced selectivity for closely related peptides and increased peak capacity compared to reversed-phase and ion-exchange columns. Methods can be developed on Promix columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems. Promix AP uses mobile phases of at least 20mM buffer concentration and a pH range from 2 to 5.5. Promix AP is available with 100Å and 300Å pore sizes.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exclusion properties for the separation of medium peptides and proteins from 1 to 10 kDa and a broad range of PI values. The Promix MP stationary phase combines a hydrophobic chain and a basic functional group for ion exclusion of positively charged molecules. Methods can be developed on Promix columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems. Promix MP uses mobile phases of at least 1mM buffer concentration and a pH range from 2 to 5.5. Promix MP is available with 300Å and 800Å pore sizes.
_ |
|
is a mixed-mode HPLC column with general hydrophobic and ion-exchange properties for the separation of small peptides under 1 kDa and PI value below 6.0. The Promix SP stationary phase combines a hydrophobic chain and an acidic functional group as a cation exchanger. The acidic functional group acts as an embedded ion-pairing reagent. Promix SP provides enhanced selectivity for closely related peptides and increased peak capacity compared to reversed-phase and ion-exchange columns. Methods can be developed on Promix columns that are fully compatible with detectors such as UV, RI, conductivity, fluorescence, evaporative light scattering (ELSD) as well as mass spectrometers (LC/MS) and preparative chromatography (prep HPLC) systems. Promix SP uses mobile phases of at least 20mM buffer concentration and a pH range from 2 to 5.5. Promix SP is available with a 100Å pore size.
_ |
|
columns are the first commercially available columns with Liquid Separation Cell technology (LiSC). With multiple patents pending, LiSC technology is based on a new chemical modification of silica gel pores that creates a liquid separation cell with its own charge characteristics, ionic strength, and hydrophobic properties. Like living cells which exist in equilibrium with the outside environment, liquid separation cells exist in constant equilibrium with the mobile phase. Obelisc R, for reversed phase, has reversed-phase character and can be used in traditional, reversed-phase type applications. Due to the presence of ionic groups and a long hydrophobic chain, Obelisc R offers additional retention and tuning that is not available with traditional reversed-phase columns. Reversed-phase separations are augmented by additional ionic and polar interactions not available on traditional reversed-phase C18 columns. Mobile phase composition changes the conformation of the long hydrophobic chain and affects the properties of the liquid separation cell and changes separation selectivity. Typical mobile phases used with Obelisc R columns are based on acetonitrile, water, and the mass spec compatible buffers ammonium formate (pH 3) and ammonium acetate (pH 5). If it is necessary to detect in low UV (<220 nm) then phosphate buffer is recommended. Obelisc R columns are compatible with mass spectrometers (LC/MS), evaporative light scattering detectors and preparative chromatography (prep HPLC) systems.
_ |
columns are the first commercially available columns with Liquid Separation Cell technology (LiSC). With multiple patents pending, LiSC technology is based on a new chemical modification of silica gel pores that creates a liquid separation cell with its own charge characteristics, ionic strength, and hydrophobic properties. Like living cells which exist in equilibrium with the outside environment, liquid separation cells exist in constant equilibrium with the mobile phase. Obelisc N, for normal phase, has very polar characteristics and works well for polar and charged analytes. In ion-exchange mode, charged analytes interact with oppositely charged groups on the stationary phase. In traditional HILIC mode, a charged or neutral polar analyte interacts with a water layer on the polar stationary phase surface. On Obelisc N the charges are greatly separated and independently accessible which results in different selectivity compared to traditional HILIC and silica columns. Mobile phase composition changes the conformation of the long hydrophilic chain. This affects the properties of the liquid separation cell and changes separation selectivity. Typical mobile phases used with Obelisc N columns are based on acetonitrile, water, and the mass spec compatible buffers ammonium formate (pH 3) and ammonium acetate (pH 5). If it is necessary to detect in low UV (<220 nm) then phosphate buffer is recommended. Obelisc N columns are compatible with mass spectrometers (LC/MS), evaporative light scattering detectors and preparative chromatography (prep HPLC) system.
_ |
|
|
