If you are looking for optimized HPLC method to analyze 1,5-Naphthalenedisulfonic Acid check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase. The spectra presented here are measured with an acidic mobile phase that has a pH of 3 or lower.

HPLC Method for Analysis of 2-Naphthalenesulfonic Acid, 1,5-Naphthalenedisulfonic Acid on BIST A Column by SIELC Technologies

2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, are two similarly-structured compounds. 2-Naphthalenesulfonic acid is commonly used in dye production and 1,5-Naphthalenedisulfonic acid is used in synthesizing the salts of basic drugs and in electrokinetic chromatography.

2-Naphthalenesulfonic Acid is an organic compound with the formula C₁₀H₇SO₃H. It is used primarily in the production of dyes, but has also historically been industrially used for a it’s various chemical reactions.

1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge. It has the chemical formula C₁₀H₈S₂O₆.

Using SIELC’s newly introduced BIST™ method, these two acids can be retained on a negatively-charged, cation-exchange BIST A. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged tartrazine analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent to minimize the formation of a solvation layer around the charged analytes. Using this new and unique analysis method, 2-Naphthalenesulfonic acid and 1,5-Naphthalenedisulfonic acid can be retained and UV detected at 270 nm.

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HPLC Method for 1,5-Naphthalenedisulfonic Acid on Newcrom B by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,5-Naphthalenedisulfonic Acid.

1,5-Naphthalenedisulfonic acid, also known as Armstrong’s acid, is a strong organic acid with a double charge. It has the chemical formula C₁₀H₈S₂O₆.

Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  1,5-Naphthalenedisulfonic Acid can be retained with great peak shape on a Newcrom B mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.

HPLC Method for 1,5-Naphthalenedisulfonic Acid on Newcrom BH by SIELC Technologies

High Performance Liquid Chromatography (HPLC) Method for Analysis of 1,5-Naphthalenedisulfonic Acid.

1,5-Naphthalenedisulfonic acid is a strong organic acid with a double charge.  Multi-charged molecules generally have a tendency to exhibit poor peak shape in HPLC on reverse-phase columns showing significant tailing.  The acid can be retained with great peak shape on a Newcrom BH mixed-mode column.  The Newcrom line of columns was designed specifically for improved peak shape of multi-charged molecules.  The method uses a mobile phase consisting of acetonitrile (ACN), water and ammonium formate buffer, making the method MS-compatible.  UV detection at 260nm can also be used.

Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).

Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.