
Naphthalenedisulfonic acid is retained by anion-exchange mechanism on Obelisc N mixed-mode HILIC column. Retention time is controlled by amount of ion in the mobile phase. Method demonstrates very good peak shape and controllable retention. This mixed-mode approach can be used for quantitation of various sulfonic acid in different matrices. UV, ELSD or LC/MS can be used for quantitation.
Sulfonic Acid 1,5-Naphthalenedisulfonic Acid
UV Detection ELSD/MS Detection

Organic acids were separated on a HILIC/cation-exchange column in HILIC/anion-exclusion mode. This column can be used for analysis of polar compounds in HILIC mode. If compounds are ionizable, additional mode of interaction can be added (cation-exchange or anion-exclusion).
3.5-dihydroxybenzoic acid Ascorbic Acid p-Toluenesulfonic Acid (PTSA) 1,5-Naphthalenedisulfonic Acid
UV Detection
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