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Separation of 4-Aminopyridine/Fampridine HPLC Separation of Vitamin C, Vitamin Group B, and Related Impurities  Vitamin C (ascorbic acid) and Vitamins Group B are separated on Obelisc N mixed-mode column. Method can be used in quantitation and determination of polar vitamins in various formulations and dietary supplements. HPLC method can be based on UV, Evaporative Light Scattering Detection (ESLD), RI or MS detection. Effect of sample matrix can be eliminated by changing mobile phase conditions. Buffer concentration, buffer pH and amount of ACN will affect every vitamin differently due to difference in polar and ionic properties. Application Analytes: Pyridoxine Vitamin B6 (Pyridoxine) 4-Aminopyridine/Fampridine Vitamin B2 (Riboflavin) 3-Aminopyridine Isonicotinic Acid Nicotinic Acid Barbituric Acid Ascorbic Acid Application Detection: UV Detection |
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