Compounds  →  4-Morpholinepropanesulfonic Acid (MOPS)

Separation of 4-Morpholinepropanesulfonic Acid (MOPS)

HPLC Separation of Morpholino Sulfates


MOPS and MES are buffers used in biology and biochemistry. They consist of a morpholine ring and propanesulfonic acid. Both molecules are zwitter-ionic and very polar. No retention can be achieved on reversed-phase columns. MOPS and MES are analyzed in HILIC mode on a Primesep N HPLC column. Other biological buffers can be analyzed with this general method. Biological buffers are not UV active and can be monitored by ELSD, CAD or LC/MS.


Application Analytes:

2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)

Application Detection:

ELSD/MS Detection
Effect of Buffer on HPLC Separation of Buffers



HEPES, CAPS, MES and MOPS are zwitterionic organic chemical buffering agents. These are very polar compounds which are not retained by traditional reverse phase chromatography. These compounds are zwitterions in nature and can be separated by mixed-mode hydrophilic interaction chromatography on Obelisc N column. Retention is achieved by combination of HILIC and ion-exchange mechanisms. These buffering agents do not have UV active groups, but can be analyzed with ESLD, LC/MS, and CAD detection.
Application Analytes:

CAPS (N-cyclohexyl-3-aminopropanesulfonic Acid)
2-Morpholinoethanesulfonic Acid (MES)
4-Morpholinepropanesulfonic Acid (MOPS)
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic Acid)

Application Detection:

ELSD/MS Detection
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