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Separation of 4-Nitrophthalic Acid HPLC Separation of 3-Nitrophthalic and 4-Nitrophthalic Acids on Primesep D HPLC Column  Nitrophthalic acid exist in the form of two isomers: 3-nitrophthalic and 4-nitrophthalic acid. Both forms have similar hydrophobicity and ionic properties. Isomers of nitrophthalic acid are separated on Primesep D column by combination of reverse phase and anion-exchange mechanisms. Method developed on a short-50 mm- column and allows fast analysis of these two isomers. Application Analytes: 3-Nitrophthalic Acid 4-Nitrophthalic Acid Application Detection: UV Detection |
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